Fifteen is such an awful(糟糕的)age, I should know. I really wish I were eighteen. If you are an adult, you can be your own boss. People like my parents and my teachers are always telling me what to do. You know what I mean. "It's time for bed, Sandy." Or "Sandy, you've watched too much TV for one night." "Sandy, stand up straight." "Sandy, you must improve your handwriting." Sandy this, Sandy that. No one tells adults when they go to bed or not to watch TV. Besides, adults get to wear what they like... Now it's going to be great to be an adult. Don't you agree?

I'm Sandy's mother. Having a fifteen-year-old daughter isn't always easy. Right now, we've got a problem. Our neigh hour, Mrs. White, wants Sandy to look after her pet dog for one week. Sandy really wants to do it, but (I         ).  She's too young and inexperienced. Well, I will never be able to relax knowing she is all alone with a dog. I've tried to explain my feelings to her, but she won't listen. She keeps telling me that the Whites live very near, so she can ask for help. She insists that Mrs. White won't ask her to look after her pet dog if she doesn't think Sandy is old enough. She says I'm "babying" her and I hate to see her mad at me. What should I do?

1. Sandy is fifteen years old and she wishes to be her own_______.

2. Sandy thinks her parents and her teachers are always telling her what to do. Give an example.

3．將文中括號內的英文句子補充完整。

4．將劃線句子She says I'm "babying" her and I hate to see her mad at me.譯成漢語。

5. Should Sandy look after Mrs. White's pet dog? Why or why not?

科學家將外源目的基因與大腸桿菌的質粒進行重組，并在大腸桿菌中成功表達。下圖表示構建重組質粒和篩選含目的基因的大腸桿菌的過程。請據圖回答：

（1）步驟①和②中常用的工具酶是                            。
（2）經過①和②步驟后，有些質粒上的                基因內插入了外源目的基因，形成重組質粒。
（3）步驟③是                                 的過程。
（4）步驟④：將三角瓶內的大腸桿菌接種到含四環素的培養基C上培養，目的是篩選     。
（5）步驟⑤:用無菌牙簽挑取C上的單個菌落，分別接種到D（含氨芐青霉素和四環素）和E（含四環素）兩個培養基的相同位置上，一段時間后，菌落的生長狀況如圖所示。含目的基因的菌落位于（選填“D”或“E”）      上。